DNA

Part:BBa_K210002

Designed by: Makoto Kashima   Group: iGEM09_Kyoto   (2009-10-09)

CEN4 and ARS1


"CEN(Centromere)・・・ With this, DNA can be distributed to sister cells.

ARS(Autonomously replicating sequence)・・・ It is yeast’s replication origin.

The vector which has CEN and ARS is duplicated one time in one cell division and distributed to sister cells. So, its copy number keeps one.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 233
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 447
    Illegal PstI site found at 233
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 262
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 233
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 233
    Illegal NgoMIV site found at 477
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None