DNA

Part:BBa_K2100017

Designed by: Kathleen Brandes   Group: iGEM16_MIT   (2016-10-14)


pENTR Taler21

Entry vector containing the TAL21 repressor gene. TAL21 is an engineered repressor from TAL effectors, amino acids which have corresponding DNA target sites. This particular TAL gene was engineered to repress the DNA sequences found on the pTAL21 promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 14
    Illegal XbaI site found at 3509
    Illegal SpeI site found at 3452
    Illegal PstI site found at 117
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 14
    Illegal SpeI site found at 3452
    Illegal PstI site found at 117
    Illegal NotI site found at 421
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 14
    Illegal BamHI site found at 3382
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 14
    Illegal XbaI site found at 3509
    Illegal SpeI site found at 3452
    Illegal PstI site found at 117
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 14
    Illegal XbaI site found at 3509
    Illegal SpeI site found at 3452
    Illegal PstI site found at 117
    Illegal NgoMIV site found at 439
    Illegal NgoMIV site found at 481
    Illegal NgoMIV site found at 2715
    Illegal AgeI site found at 32
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/part_collection/2016
Parameters
None