DNA

Part:BBa_K2100016

Designed by: Kathleen Brandes   Group: iGEM16_MIT   (2016-10-14)


pENTR Taler14

Entry vector containing the TAL14 repressor gene. TAL14 is an engineered repressor from TAL effectors, amino acids which have corresponding DNA target sites. This particular TAL gene was engineered to repress the DNA sequences found on the pTAL14 promoter.


Sequence and Features

Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 14
    Illegal XbaI site found at 3305
    Illegal SpeI site found at 3248
    Illegal PstI site found at 117
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 14
    Illegal SpeI site found at 3248
    Illegal PstI site found at 117
    Illegal NotI site found at 421
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 14
    Illegal BamHI site found at 3178
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 14
    Illegal XbaI site found at 3305
    Illegal SpeI site found at 3248
    Illegal PstI site found at 117
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 14
    Illegal XbaI site found at 3305
    Illegal SpeI site found at 3248
    Illegal PstI site found at 117
    Illegal NgoMIV site found at 439
    Illegal NgoMIV site found at 481
    Illegal NgoMIV site found at 2511
    Illegal AgeI site found at 32
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal EcoRI site found at 14
    Illegal XbaI site found at 3305
    Illegal SpeI site found at 3248
    Illegal PstI site found at 117


[edit]
Categories
//awards/part_collection/2016
Parameters
None