DNA
Part:BBa_K2100012
Designed by: Kathleen Brandes Group: iGEM16_MIT (2016-10-10)
pEXPR hef1a-mKate
mKate is a red fluorescent protein constituitively expressed with the hEF1a (human elongation factor 1 alpha) promoter.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 6
Illegal EcoRI site found at 1210
Illegal PstI site found at 337
Illegal PstI site found at 842 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 6
Illegal EcoRI site found at 1210
Illegal PstI site found at 337
Illegal PstI site found at 842 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 6
Illegal EcoRI site found at 1210
Illegal BglII site found at 591
Illegal BamHI site found at 1197
Illegal XhoI site found at 990 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 6
Illegal EcoRI site found at 1210
Illegal PstI site found at 337
Illegal PstI site found at 842 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 6
Illegal EcoRI site found at 1210
Illegal PstI site found at 337
Illegal PstI site found at 842
Illegal NgoMIV site found at 725
Illegal AgeI site found at 103 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1873
Illegal SapI.rc site found at 1255
[edit]
Categories
Parameters
//awards/part_collection/2016
None |