Coding

Part:BBa_K210001

Designed by: Makoto Kashima   Group: iGEM09_Kyoto   (2009-10-09)


lacI repressor with kozak sequence.

This part is derived from E.coli's lacI repressor. By PCR, we exchanged ATG to kozak sequence. We used this in order to express lacI in yeast.

Sequence and Features


Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1158
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
Parameters
None