Composite

Part:BBa_K2097002

Designed by: Prachi Shah   Group: iGEM16_Austin_UTexas   (2016-10-14)


Yellow-green chromoprotein with medium promoter and RBS

This is a composite part made up of BBa_K1033916 (amajLime, a yellow-green chromoprotein) and BBa_K608006 (a medium promoter and RBS). It is made up of a medium promoter, BBa_J23110, and a medium RBS, BBa_B0032. Served as the control group for the part BBa_K2097000.

Usage and Biology

BBa_K1033916 was created by Uppasala 2013. It comes from the coral Anemonia majano. BBa_K608006 was created by Freiburg 2011.

Figure 1. amajLime expressed in E. coli in liquid LB
Figure 2. amajLime expressed in E. coli after being spun down in a microcentrifuge




















Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None