Composite

Part:BBa_K2097001

Designed by: Prachi Shah   Group: iGEM16_Austin_UTexas   (2016-10-14)


Blue chromoprotein with strong promoter and RBS

This is a composite part made up of BBa_K592009 (blue chromoprotein) and BBa_K608002 (a strong RBS and promoter). The protein exhibits a strong blue/purple color when expressed. BBa_K608002 is made up of a promoter, BBa_J23104, and an RBS, BBa_B0034.

Usage and Biology

BBa_K592009 was created by Uppsala-Sweden 2011. It comes from the coral Acropora millepora, amilCP. BBa_K608002 was created by Freiburg 2011.

Figure 1. amilCP expressed in E. coli and on an LB/agar plate
Figure 2. amilCP expressed in E. coli and in liquid LB


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None