Reporter

Part:BBa_K2095003

Designed by: Liu iGEM 2016   Group: iGEM16_Linkoping_Sweden   (2016-10-10)


mRFP1 regulated by pLIP

This composite part was designed by the Linkoping iGEM team 2016 to demonstrate the functionality of the light-inducible promoter, LIP, Biobrick (BBa_K2095000) A synthesised truncated version of the promotor of the light-inducible protein gene (LIP) in Dunaliella microalgae (1,2) was set to control the expression of the mRFP1 reporter gene, obtained from BBa_E1010. This part is terminated by a RuBisCO terminator (BBa_K2095002).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 866
    Illegal AgeI site found at 978
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1. Park S, Lee Y, Lee J-H, Jin E. Expression of the high light-inducible Dunaliella LIP promoter in Chlamydomonas reinhardtii. Planta [Internet]. 2013 Dec [cited 2016 Sep 4];238(6):1147–56. Available from: http://www.ncbi.nlm.nih.gov/pubmed/24043576

2. Baek K, Lee Y, Nam O, Park S, Sim SJ, Jin E. Introducing Dunaliella LIP promoter containing light-inducible motifs improves transgenic expression in Chlamydomonas reinhardtii. Biotechnol J. 2016;11(3):384–92.

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Parameters
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