Part:BBa_K209496

AarI B-D part, FRB

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

2018 Peking Team improvement

Team Peking 2018 improved this part. Please view http://2018.igem.org/Team:Peking/Improve for more details.

Firstly, we fused this this part with yEGFP, which made it visible in the yeast through fluorescent microscope.

https://parts.igem.org/Part:BBa_K2601007

Then we characterized the expression of Frb-yEGFP with 2 promoters pTet07 and PDH3. The expression of these promoters was measured through flow cytometry.

https://parts.igem.org/Part:BBa_K2601021; https://parts.igem.org/Part:BBa_K2601023

Moreover, we fused Frb-yEGFP with HOtag6. This part can form phase separation with FKBP-yEGFP-HOTag3 in the presence of rapamycin.

https://parts.igem.org/Part:BBa_K2601010

Finally, we characterized the expression of Frb-yEGFP-HOTag6 with 3 promoters pTet07, pTEF1 and PDH3. The expression of these promoters was measured through flow cytometry.

https://parts.igem.org/Part:BBa_K2601032; https://parts.igem.org/Part:BBa_K2601033; https://parts.igem.org/Part:BBa_K2601034