Part:BBa_K209486

AarI B-D part, DOR-Actinin1ABD

delta Opioid receptor fused to the actin binding domain of alpha-actinin-1. The fusion is flanked by AarI BD sites.

Usage and Biology

The ABD of ACTN1 binds to the C-terminus of DOR, and has been shown to modulate the recycling of the receptor. Unpublished results suggest that a direct fusion of these proteins shows improved recycling over wt DOR.

References: Actin-binding protein alpha-actinin-1 interacts with the metabotropic glutamate receptor type 5b and modulates the cell surface expression and function of the receptor. Cabello N, Remelli R, Canela L, Soriguera A, Mallol J, Canela EI, Robbins MJ, Lluis C, Franco R, McIlhinney RA, Ciruela F. J Biol Chem. 2007 Apr 20;282(16):12143-53. Epub 2007 Feb 20.

Engineered protein connectivity to actin mimics PDZ-dependent recycling of G protein-coupled receptors but not its regulation by Hrs. Lauffer BE, Chen S, Melero C, Kortemme T, von Zastrow M, Vargas GA. J Biol Chem. 2009 Jan 23;284(4):2448-58. Epub 2008 Nov 10.

Documentation and characterization

This part is described in the following experiment:

http://2009.igem.org/Team:UCSF/NavigationPart2

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 31
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 31
  Illegal NotI site found at 1010
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 641
  Illegal BglII site found at 1286
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 31
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 31
  
• 1000
  COMPATIBLE WITH RFC[1000]