Composite

Part:BBa_K209480

Designed by: Katja Kolar   Group: iGEM09_UCSF   (2009-10-05)


pcDNA-SSFYFP-hM4D-Villin headpiece



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 952
    Illegal XbaI site found at 1065
    Illegal SpeI site found at 249
    Illegal SpeI site found at 935
    Illegal PstI site found at 957
    Illegal PstI site found at 2387
    Illegal PstI site found at 5854
    Illegal PstI site found at 6558
    Illegal PstI site found at 7353
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 952
    Illegal NheI site found at 895
    Illegal NheI site found at 7589
    Illegal SpeI site found at 249
    Illegal SpeI site found at 935
    Illegal PstI site found at 957
    Illegal PstI site found at 2387
    Illegal PstI site found at 5854
    Illegal PstI site found at 6558
    Illegal PstI site found at 7353
    Illegal NotI site found at 978
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 952
    Illegal BglII site found at 12
    Illegal BglII site found at 5636
    Illegal BglII site found at 7836
    Illegal BamHI site found at 929
    Illegal XhoI site found at 1059
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 952
    Illegal XbaI site found at 1065
    Illegal SpeI site found at 249
    Illegal SpeI site found at 935
    Illegal PstI site found at 957
    Illegal PstI site found at 2387
    Illegal PstI site found at 5854
    Illegal PstI site found at 6558
    Illegal PstI site found at 7353
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 952
    Illegal XbaI site found at 1065
    Illegal SpeI site found at 249
    Illegal SpeI site found at 935
    Illegal PstI site found at 957
    Illegal PstI site found at 2387
    Illegal PstI site found at 5854
    Illegal PstI site found at 6558
    Illegal PstI site found at 7353
    Illegal NgoMIV site found at 1497
    Illegal NgoMIV site found at 2838
    Illegal NgoMIV site found at 3121
    Illegal NgoMIV site found at 7460
    Illegal AgeI site found at 1013
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5592
    Illegal BsaI.rc site found at 882
    Illegal BsaI.rc site found at 4646
    Illegal SapI site found at 3566
    Illegal SapI.rc site found at 2687
    Illegal SapI.rc site found at 2897


[edit]
Categories
Parameters
None