Coding

Part:BBa_K209472

Designed by: Katja Kolar   Group: iGEM09_UCSF   (2009-10-02)


AarI B-D part, ADRA2A (B2AR)

This is the coding sequence of Homo sapiens adrenoceptor beta 2 (ADRB2). This part has been improved by SJTU-Biox-Shanghai 2016 iGEM team to generate two parts with modified sequence: BBa_K1969001 and BBa_K1969002.

BBa_K1969001 Group: SJTU-Biox-Shanghai 2016 Author: Yijie Lai Summary: This improved version using site mutation to eliminate the illegal PstI site by changing G at 192 bp to A, and was tested for epinephrine response when expressed in yeast Saccharomyces cerevisiae under two different promoters, GAL1 and ADH1.

BBa_K1969002 Group: SJTU-Biox-Shanghai 2016 Author: Yijie Lai Summary: This improved version using site mutation to eliminate the illegal PstI site by changing G at 192 bp to A and adding a his-tag to its C terminus. The sequence was tested for membrane localization when expressed in yeast Saccharomyces cerevisiae under two different promoters, GAL1 and ADH1.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 190
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 190
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 778
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 190
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 190
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 364
    Illegal BsaI.rc site found at 562


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