Composite

Part:BBa_K209469

Designed by: Katja Kolar   Group: iGEM09_UCSF   (2009-10-05)


pCEP-SSFYFP-hM4D-Beta Pix



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6034
    Illegal EcoRI site found at 8330
    Illegal EcoRI site found at 8726
    Illegal XbaI site found at 609
    Illegal XbaI site found at 1146
    Illegal XbaI site found at 12992
    Illegal XbaI site found at 14414
    Illegal SpeI site found at 18
    Illegal SpeI site found at 2057
    Illegal PstI site found at 6786
    Illegal PstI site found at 8423
    Illegal PstI site found at 8816
    Illegal PstI site found at 10083
    Illegal PstI site found at 10612
    Illegal PstI site found at 11316
    Illegal PstI site found at 12111
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6034
    Illegal EcoRI site found at 8330
    Illegal EcoRI site found at 8726
    Illegal NheI site found at 630
    Illegal NheI site found at 642
    Illegal NheI site found at 12347
    Illegal SpeI site found at 18
    Illegal SpeI site found at 2057
    Illegal PstI site found at 6786
    Illegal PstI site found at 8423
    Illegal PstI site found at 8816
    Illegal PstI site found at 10083
    Illegal PstI site found at 10612
    Illegal PstI site found at 11316
    Illegal PstI site found at 12111
    Illegal NotI site found at 646
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6034
    Illegal EcoRI site found at 8330
    Illegal EcoRI site found at 8726
    Illegal BglII site found at 604
    Illegal BglII site found at 10111
    Illegal BglII site found at 10394
    Illegal BamHI site found at 745
    Illegal XhoI site found at 727
    Illegal XhoI site found at 13296
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6034
    Illegal EcoRI site found at 8330
    Illegal EcoRI site found at 8726
    Illegal XbaI site found at 609
    Illegal XbaI site found at 1146
    Illegal XbaI site found at 12992
    Illegal XbaI site found at 14414
    Illegal SpeI site found at 18
    Illegal SpeI site found at 2057
    Illegal PstI site found at 6786
    Illegal PstI site found at 8423
    Illegal PstI site found at 8816
    Illegal PstI site found at 10083
    Illegal PstI site found at 10612
    Illegal PstI site found at 11316
    Illegal PstI site found at 12111
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6034
    Illegal EcoRI site found at 8330
    Illegal EcoRI site found at 8726
    Illegal XbaI site found at 609
    Illegal XbaI site found at 1146
    Illegal XbaI site found at 12992
    Illegal XbaI site found at 14414
    Illegal SpeI site found at 18
    Illegal SpeI site found at 2057
    Illegal PstI site found at 6786
    Illegal PstI site found at 8423
    Illegal PstI site found at 8816
    Illegal PstI site found at 10083
    Illegal PstI site found at 10612
    Illegal PstI site found at 11316
    Illegal PstI site found at 12111
    Illegal NgoMIV site found at 733
    Illegal NgoMIV site found at 12218
    Illegal AgeI site found at 681
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5402
    Illegal BsaI site found at 6960
    Illegal BsaI site found at 10350
    Illegal BsaI.rc site found at 4440
    Illegal BsaI.rc site found at 9660
    Illegal BsaI.rc site found at 13197
    Illegal BsaI.rc site found at 14499
    Illegal SapI.rc site found at 12803


[edit]
Categories
Parameters
n/aAarI A-B part, signal sequence-FLAG-YFP [SSFYFP]