Composite

Part:BBa_K209457

Designed by: Katja Kolar   Group: iGEM09_UCSF   (2009-10-05)


pEF1-SSFYFP-hM4D-Vav



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 346
    Illegal PstI site found at 1050
    Illegal PstI site found at 1845
    Illegal PstI site found at 2960
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2081
    Illegal PstI site found at 346
    Illegal PstI site found at 1050
    Illegal PstI site found at 1845
    Illegal PstI site found at 2960
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 128
    Illegal BglII site found at 2626
    Illegal BglII site found at 4582
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 346
    Illegal PstI site found at 1050
    Illegal PstI site found at 1845
    Illegal PstI site found at 2960
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 346
    Illegal PstI site found at 1050
    Illegal PstI site found at 1845
    Illegal PstI site found at 2960
    Illegal NgoMIV site found at 1952
    Illegal NgoMIV site found at 3313
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 84
    Illegal BsaI.rc site found at 3490
    Illegal BsaI.rc site found at 3684
    Illegal BsaI.rc site found at 4498
    Illegal SapI site found at 2387


[edit]
Categories
Parameters
None