Composite

Part:BBa_K209456

Designed by: Katja Kolar   Group: iGEM09_UCSF   (2009-10-05)


pEF1-SSFYFP-hM4D-LPD(775-1250)



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 346
    Illegal PstI site found at 1050
    Illegal PstI site found at 1845
    Illegal PstI site found at 2673
    Illegal PstI site found at 3048
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2081
    Illegal PstI site found at 346
    Illegal PstI site found at 1050
    Illegal PstI site found at 1845
    Illegal PstI site found at 2673
    Illegal PstI site found at 3048
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 128
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 346
    Illegal PstI site found at 1050
    Illegal PstI site found at 1845
    Illegal PstI site found at 2673
    Illegal PstI site found at 3048
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 346
    Illegal PstI site found at 1050
    Illegal PstI site found at 1845
    Illegal PstI site found at 2673
    Illegal PstI site found at 3048
    Illegal NgoMIV site found at 1952
    Illegal AgeI site found at 2554
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 84
    Illegal BsaI site found at 3018
    Illegal BsaI.rc site found at 2965
    Illegal BsaI.rc site found at 3032
    Illegal BsaI.rc site found at 3705


[edit]
Categories
Parameters
None