Plasmid

Part:BBa_K209399

Designed by: Oliver Hoeller   Group: iGEM09_UCSF   (2009-09-14)

AarI A-D acceptor vector

Aar1 A-D acceptor vector, based on pDONR221, Used to assemble A-B,B-C and C-D fragments in 1 step


Documentation and Characterization

for further details on how to use this vector please refer to RFC28


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 721
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 239
    Illegal NheI site found at 505
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 706
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 721
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 721
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 116


[edit]
Categories
Parameters
n/aAarI A-D acceptor vector