Part:BBa_K2092008

Pcon (J23110) + RBS (B0034) + alcR

This part encodes for the alcR gene, which is a positive regulatory gene in the ethanol regulon of filamentous fungus Aspergillus nidulans (A.nidulans). It encodes the AlcR protein, an ethanol-activated transcription factor which would bind to its target promoters, including the alcA (PalcA, https://parts.igem.org/Part:BBa_K2092002) and aldA promoters.

AlcR is also known as a zinc binuclear cluster activator as it contains a DNA-binding domain belonging to the C6 zinc binuclear cluster family. AlcR is unique in that it can bind to symmetric and asymmetric DNA sites with the same apparent affinity and also bind to single site with high affinity [1].

A constitutive promoter (https://parts.igem.org/Part:BBa_K2092007) and strong RBS (Part BBa_J34801, https://parts.igem.org/Part:BBa_J34801) has been added upstream of the start codon.

Usage and Biology

This part is useful to measure the promoter strength of alcA/aldA promoters.

Reference

[1] Felenbok, B., Sequeval, D., Mathieu, M., Sibley, S., Gwynne, D.I. and Davies, R.W. (1988). The ethanol regulon in Aspergillus nidulans: Characterization and sequence of the positive regulatory gene alcR. Gene, 73(2), 385–396.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2611
  Illegal BamHI site found at 1615
  Illegal BamHI site found at 2353
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1319
  Illegal AgeI site found at 1447
  Illegal AgeI site found at 1978
  
• 1000
  COMPATIBLE WITH RFC[1000]