Part:BBa_K2092000

AOx

This part encodes for the AOx1 protein from Pichia pastoris. 

Usage and Biology

Alcohol oxidase is an oligomeric enzyme with eight identical sub-units, containing a bound molecule cofactor, flavin adenine dinucleotide (FAD). It is produced by methylotrophic yeasts (such us our chosen Pichia) located and assembled in peroxisomes during growth on methanol (1). AOx is implicated in the methanol oxidation pathway in yeasts, however it can also oxidise other short-chain alcohols, such as ethanol, propanol or butanol (2).

AOX is thus responsible for the oxidation of primary alcohols to the equivalent aldehyde, using molecular oxygen (O2) as the electron acceptor, creating Hydrogen Peroxide (H202) as a by-product.

References

[2]Azevedo, A. M., Prazeres, D. M. F., Cabral, J. M., & Fonseca, L. P. (2005). Ethanol biosensors based on alcohol oxidase. Biosensors and Bioelectronics,21(2), 235-247.

[2] Maleknia, S., Ahmadi, H., & Norouzian, D. (2011). Immobilization of Pichia pastoris cells containing alcohol oxidase activity. Iranian journal of microbiology, 3(4), 210.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 363
  Illegal BglII site found at 540
  Illegal BamHI site found at 1305
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 595
  Illegal AgeI site found at 1045
  Illegal AgeI site found at 1173
  
• 1000
  COMPATIBLE WITH RFC[1000]