Coding
Part:BBa_K2091007
Designed by: I. Bariah, E. Zajfman, I. Segal Group: iGEM16_BGU_ISRAEL (2016-10-03)
LC-Cutinase Variant R4
This is a LC-Cutinase mutated protein. It has 4 amino acid mutations compared to the codon optimized protein. The mutations are restricted to a specific distance from the active site of the protein.
Characterization
The LC-Cutinase variants were grown on M9 minimal medium plates with shredded PET pellets as a sole carbon source to test their ability to degrade PET.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |