Coding

Part:BBa_K2091007

Designed by: I. Bariah, E. Zajfman, I. Segal   Group: iGEM16_BGU_ISRAEL   (2016-10-03)


LC-Cutinase Variant R4

This is a LC-Cutinase mutated protein. It has 4 amino acid mutations compared to the codon optimized protein. The mutations are restricted to a specific distance from the active site of the protein.

Figure 1: A 3D model of the R4 protein.


















Characterization

The LC-Cutinase variants were grown on M9 minimal medium plates with shredded PET pellets as a sole carbon source to test their ability to degrade PET.

Figure 2: LC-Cutinase R4 variant grown on a M9 agar plate with shredded PET pellets as a sole carbon source. Colonies marked in red.
Figure 3: Control. E. coli expressing the W.T. LC-Cutinase grown on a M9 plate with no carbon source.





























Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None