Part:BBa_K2091002

4-Carboxymuconolactone Decarboxylase

pcaC is a gene encoding the 4-carboxymuconolactone decarboxylase protein from Pseudomonas putida strain KT2440.

Usage and Biology

4-carboxymuconolactone decarboxylase is the enzyme the catalyzes the conversion of 2-carboxy-2,5-dihydro-5-oxofuran-2-acetate to 3-oxoadipate enol lactone and carbon dioxide.
The enzyme has a broad optimum of pH, between 6.5 to 8.0, and and estimated molecular weight of 93,000 Daltons.
4-carboxymuconolactone decarboxylase belongs to the family of carboxy-lyases, enzymes which remove a carboxyl group from their substrate. The reaction carried out by this enzyme is a decarboxylation reaction in which a carbon-carbon bond is cleaved and CO2 is released.
This enzyme catalyzes a reaction that is part of the Protocatachuate degradation pathway, in which protocatechuate is converted to 3-oxoadipate.

Figure 1: The enzymatic reaction catalyzed by the 4-carboxymuconolactone decarboxylase enzyme from P. putida.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]