Protocatechuate 3,4-dioxygenase Beta Subunit
pcaH is a gene encoding for the Beta subunit of the Protocatechuate 3,4-dioxygenase enzyme from Pseudomonas putida.
Usage and Biology
Protocatechuate 3,4-dioxygenase catalyzes the convertion of 3,4-dihydroxybenzoic acid and O2 to 3-carboxy-cis,cis-muconate. Dioxygenases are oxydoreductase enzymes. They utilize a dioxygen (O2) molecule as an oxidant. As opposed to monooxygenases, in dioxygenases reactions both atoms from O2 are incorporated into the substrate. Protocatechuate dioxygenase from P. putida has a molecular weight of 200,000 daltons and contains 4 alpha subunits of 23,000 daltons, 4 beta subunits of 26,500 daltons, and 4 ferric irons, suggesting that the enzyme is a tetramer of (𝛂𝛃Fe3+) catalytic units.
The ability of P. putida to grow on protocatechuate (3,4-dihydroxybenzoate) was tested using an assay of M9 minimal medium with varying concentrations of protocatechuate as a sole carbon source (figure 2,3).
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NotI site found at 317
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25Illegal NgoMIV site found at 244
Illegal NgoMIV site found at 313
- 1000COMPATIBLE WITH RFC