Part:BBa_K209091

ACA-linker-SHIP1

ACA localizes to the back of the cell fused to the catalytic domain of SHIP1 (a 5'phosphatase).

Documentation and Characterization

for details please see the UCSF team 2009 Wiki: [http://2009.igem.org/Matrix_of_Fusion_Constructs] Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1408
  Illegal XbaI site found at 3922
  Illegal PstI site found at 4360
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1408
  Illegal PstI site found at 4360
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1408
  Illegal BglII site found at 3145
  Illegal BglII site found at 4074
  Illegal XhoI site found at 5457
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1408
  Illegal XbaI site found at 3922
  Illegal PstI site found at 4360
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1408
  Illegal XbaI site found at 3922
  Illegal PstI site found at 4360
  
• 1000
  COMPATIBLE WITH RFC[1000]