Composite

Part:BBa_K209069

Designed by: Ryan Quan   Group: iGEM09_UCSF   (2009-10-12)

PTEN-linker-NFA

Dictyostelium PTEN (a 3' phospahatase) localizing to the back of cells, binding to PI(4,5)P2 fused to the catalytic domain of Dictyostelium NFA (a Ras GAP)


Documentation and Characterization

for details please see the UCSF team 2009 Wiki: [http://2009.igem.org/Matrix_of_Fusion_Constructs]

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1847
    Illegal PstI site found at 2205
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1847
    Illegal PstI site found at 2205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1847
    Illegal BamHI site found at 744
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1847
    Illegal PstI site found at 2205
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1847
    Illegal PstI site found at 2205
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1138


[edit]
Categories
Parameters
n/aPTEN-linker-NFA