Composite

Part:BBa_K209066

Designed by: Ryan Quan   Group: iGEM09_UCSF   (2009-10-12)

PTEN-linker-RasC da

Dictyostelium PTEN (a 3' phospahatase) localizing to the back of Dictyostelium cells, binding to PI(4,5)P2 fused to Dictyostelium RasC (dominant active version (G13T) without its C terminal CAAX lipid anchoring motif)


References:

Tumor suppressor PTEN mediates sensing of chemoattractant gradients. Iijima M, Devreotes P. Cell. 2002 May 31;109(5):599-610.

Localized Ras signaling at the leading edge regulates PI3K, cell polarity, and directional cell movement. Sasaki AT, Chun C, Takeda K, Firtel RA. J Cell Biol. 2004 Nov 8;167(3):505-18.

Documentation and Characterization

for details please see the UCSF team 2009 Wiki: [http://2009.igem.org/Matrix_of_Fusion_Constructs]

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2072
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 744
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2072
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2072
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1138


[edit]
Categories
Parameters
n/aPTEN-linker-RasC da