Composite

Part:BBa_K209063

Designed by: Ryan Quan   Group: iGEM09_UCSF   (2009-10-12)

PTEN-linker-PI3K(-N)

Dictyostelium PTEN (a 3' phospahatase) localizing to the back of cells, binding to PI(4,5)P2 fused to Dictyostelium PI3K1 without the N terminal localization domain

Documentation and Characterization

for details please see the UCSF team 2009 Wiki: [http://2009.igem.org/Matrix_of_Fusion_Constructs]

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3754
    Illegal SpeI site found at 1828
    Illegal PstI site found at 3902
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3754
    Illegal SpeI site found at 1828
    Illegal PstI site found at 3902
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3754
    Illegal BamHI site found at 744
    Illegal BamHI site found at 4755
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3754
    Illegal SpeI site found at 1828
    Illegal PstI site found at 3902
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3754
    Illegal SpeI site found at 1828
    Illegal PstI site found at 3902
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1138


[edit]
Categories
Parameters
n/aPTEN-linker-PI3K(-N)