Composite

Part:BBa_K209036

Designed by: Ryan Quan   Group: iGEM09_UCSF   (2009-10-12)

PLC-PH-linker-SHIP1

the PI(4,5)P2 binding PH domain of Phospholipase C fused to the catalytic domain of SHIP1 (a 5' phosphatase) acting on PI(3,4,5)P3


Documentation and Characterization

for details please see the UCSF team 2009 Wiki: [http://2009.igem.org/Matrix_of_Fusion_Constructs]

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 527
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 527
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1624
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 527
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 527
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aPLC-PH-linker-SHIP1