copA + riboJ +mhbDHIM + terminator
The composite part contains copA promoter, riboJ , a gene cluster mhbDHIM , and a terminator. The promoter is induced by copper ion and transcribe downstream genes. The riboJ between copA and the gene cluster can decrease interference of one part type （promoter）on another（protein coding sequence）. The gene cluster mhbDHIM contains four connected genes which can degrade 3-hydroxybenzoate to the Fumarate and Pyruvate step by step.
After experienting, we found that the device can degrade the 3-hydroxybenzoate completely in short time. Also, engineering bacteria with the device grows better in the medium with 3-hydroxybenzoate than Klebsiella pneumoniae M5a1which is capable of utilizing 3-hydroxybenzoate via gentisate, and can tolerate higher concentration of 3- hydroxybenzoate.In conclusion, the device can help the E.coli degrade the 3- hydroxybenzoate effectively.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21INCOMPATIBLE WITH RFCIllegal BamHI site found at 2518
Illegal BamHI site found at 2904
- 23COMPATIBLE WITH RFC
- 25INCOMPATIBLE WITH RFCIllegal NgoMIV site found at 480
Illegal NgoMIV site found at 754
Illegal NgoMIV site found at 2708
Illegal NgoMIV site found at 3845
Illegal AgeI site found at 816
- 1000INCOMPATIBLE WITH RFCIllegal BsaI.rc site found at 3038