Designed by: Yuxin He Group: iGEM16_NAU-CHINA (2016-10-09)
This composite part includes a constitutive promoter (BBa_J23100), a RBS(BBa_B0034), a putative 3-hydroxybenzoate transporter gene(mhbT) and the double terminator (:BBa_B0015). The gene mhbT encodes a transport protein which is located on the cytomembrane. It can transport the 3-HBA into the cell.
This composite part can be used in degradation system of 3-hydroxybenzoate (3-HBA), and is also helpful in induction system whose derivant is 3-HBA.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12INCOMPATIBLE WITH RFCIllegal NheI site found at 7
Illegal NheI site found at 30
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25INCOMPATIBLE WITH RFCIllegal NgoMIV site found at 227
- 1000COMPATIBLE WITH RFC