Coding
T

Part:BBa_K2088666

Designed by: Shi Kejian   Group: iGEM16_NAU-CHINA   (2016-10-07)


MhbT is responsible for the uptake of 3-hydroxybenzoate

MhbT, a transport protein which is located on the cytomembrane, can transport the 3-HBA into the cell. It can be used in degradation system of 3-HBA, and can also be used in induction system whose derivant is 3-HBA.

According to sequence alignment, MhbT exhibited 42% identity with the 4-hydroxybenzoate and protocatechuate transporter PcaK from P.putida, 27% identity with the benzoate transporter BenK from Acinetobacter sp. Using the MhbT-GFP fusion proteins , we can find that MhbT is located to the periphery of cells.

Figure1: Localization of green fluorescent proteins by confocal microscopy. (A1, B1, and C1) GFP expressed in K. pneumoniae M5a1[pZWXY005] (A1), P. putida PaW340[pZWXY005] (B1), and E. coli BL21(DE3) harboring plasmid pGFPe (C1) (controls), showing that GFP was distributed throughout the cytoplasm. (A2, B2, and C2) MhbT-GFP expressed in K. pneumoniae M5a1[pZWXY004](A2), P. putida PaW340[pZWXY004] (B2), and E. coli BL21(DE3)[pZWXY006] (C2), showing that the fusion proteins were all located at the periphery of the cells.[1]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 166
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
//cds
Parameters
protein