Designed by: Shi Kejian Group: iGEM16_NAU-CHINA (2016-10-07)
MhbT is responsible for the uptake of 3-hydroxybenzoate
MhbT, a transport protein which is located on the cytomembrane, can transport the 3-HBA into the cell. It can be used in degradation system of 3-HBA, and can also be used in induction system whose derivant is 3-HBA.
According to sequence alignment, MhbT exhibited 42% identity with the 4-hydroxybenzoate and protocatechuate transporter PcaK from P.putida, 27% identity with the benzoate transporter BenK from Acinetobacter sp. Using the MhbT-GFP fusion proteins , we can find that MhbT is located to the periphery of cells.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25INCOMPATIBLE WITH RFCIllegal NgoMIV site found at 166
- 1000COMPATIBLE WITH RFC