Designed by: Shi Kejian Group: iGEM16_NAU-CHINA (2016-10-08)
A device that expresses the XylE- catechol 2,3-dioxygenase to degrade the catechol .
In this device, promoter J23100 and the RBS B0034 are connected before the XylE - catechol 2,3-dioxygenase.
Catechol is one of the main metabolites during the degradation of aromatic compounds. This device is useful to degrade catechol in some degradation systems to reduce the toxicity of catechol to cells. With the strong promoter and rbs, xylE can convert to 2-hydroxymuconate semialdehyde, a bright yellow product which can be metabolized by E.coli.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12INCOMPATIBLE WITH RFCIllegal NheI site found at 7
Illegal NheI site found at 30
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25INCOMPATIBLE WITH RFCIllegal NgoMIV site found at 400
Illegal NgoMIV site found at 572
Illegal AgeI site found at 923
- 1000COMPATIBLE WITH RFC
- We verified the function of xylE by constructing a device BBa_K2088010.