Intermediate
Part:BBa_K2088009
Designed by: Xia Chen Group: iGEM16_NAU-CHINA (2016-10-08)
nativeRBS-C23O with double Terminator
This part includes catechol 2,3-dioxygenase(C23O) gene from Sphingobium sp. YBL2 with native RBS and a double Terminator(B0015). This part functions as both a reporter and a degradation gene, since C23O converts catechol to 2-hydroxymuconate semialdehyde, which shows a bright yellow color. When ligated with another gene or an unknown promoter, this part can be used to test the expression of gene and the strength of promoter just utilizing catechol solution as "chromogenic reagent".
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 839
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Categories
Parameters
None |