Part:BBa_K2088003

pbaA1A2

PbaA1A2 protein coding region. This part encodes an oxygenase, which is one of three components of the 3-phenoxybenzoate 1',2'-dioxygenase.

3-phenoxybenzoate 1',2'-dioxygenase is an angular dioxygenase. It belongs to a type IV Rieske non-heme iron aromatic ring-hydroxylating oxygenase (RHO) system consisting of a hetero-oligomeric oxygenase, a [2Fe-2S]-type ferredoxin, and a GR-type reductase. In our project, 3-phenoxybenzoate 1',2'-dioxygenase catalyzes the hydroxylation in the 1'and 2' positions of the benzene moiety of 3-phenoxybenzoate(3-PBA), yielding 3-hydroxybenzoate(3-HBA) and catechol. PbaA1A2 is the terminal oxidase during the angular dioxygenation of 3-PBA.

pbaA1 encodes a putative 48-kDa protein consisting of 435 amino acids, pbaA2 encodes a putative 21-kDa protein consisting of 176 amino acids. They encodes phenoxybenzoic acid dioxygenase alpha subunit and beta subunit respectively.

PbaA1 contains conserved sequences for a Rieske [2Fe-2S] domain (CXHX17CX2H) and a nonheme Fe(II) domain (EX4DX2HX4H), suggesting that PbaA1 is the oxygenase component of an RHO.

Note that this part should always be used with:

• pbaB ：a [2Fe-2S]-type ferredoxin. For more details, see BBa_K2088007.
• pbaC：a glutathione reductase (GR)-type reductase to physiologically transfer electrons to PbaA1A2B. For more details ,see BBa_K2088005.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 215
  Illegal NgoMIV site found at 822
  Illegal NgoMIV site found at 846
  Illegal AgeI site found at 730
  
• 1000
  COMPATIBLE WITH RFC[1000]