Designed by: Xia Chen Group: iGEM16_NAU-CHINA (2016-09-11)
catechol 2,3-dioxygenase gene
This basic part is catechol 2,3-dioxygenase(C23O) gene from Sphingobium sp. YBL2. It converts catechol by meta-cleavage to the bright yellow product 2-hydroxymuconate semialdehyde. In our project, it plays a great role of degrading catechol, which is one of the main metabolites in 3-phenoxybenzoate degradation.
We teseted this basic part by constrcuting two devices:
- 1.Constitutive promoter (BBa_J23100) + its native rbs + C23O + double terminator (BBa_B0015).
- 2.Constitutive promoter (BBa_J23100) + BBa_B0034 + C23O + double terminator (BBa_B0015).
We made plate streaking for three E.coli with different plasmids.
Figure1 shows the plate before dripping catechol solution.
Figure2 shows the plate after dripping catechol solution(0.2mol/L).
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000INCOMPATIBLE WITH RFCIllegal SapI site found at 804
|biology||Sphingobium sp. YBL2|