Part:BBa_K2083012

Wild Type Human Beta Globin Fused to GFP Reporter

Coding region for human beta globin with GFP reporter fused to the end. GFP fused to beta globin with a premature termination codon (PTC) can be found at BBa K2083011.

Figure 1: Wild Type Globin Fluorescence

Figure 2: PTC Globin Fluorescence

The validation of the globin reporters followed the same procedures as the ATG/ACG reporters. The wild type PTC globin reporter showed a much lower level of expression compared to the ATG reporter (BBa_K2083009) (14.77% of positive control).

Figure 3: Efficiency of WT/PTC β-globin reporters compared to positive control. Error bars represent standard error between normalized results from 3 biological replicates of the experiment.

For how the values were calculated see this: https://static.igem.org/mediawiki/2016/c/c7/T--WPI_Worcester--Imaging_Analysis.pdf

For the fluorescence values see here:

https://static.igem.org/mediawiki/2016/4/45/T--WPI_Worcester--globin_Trial1.pdf: Trial 1

https://static.igem.org/mediawiki/2016/8/89/T--WPI_Worcester--globin_Trial2.pdf: Trial 2

https://static.igem.org/mediawiki/2016/3/3d/T--WPI_Worcester--globin_Trial3.pdf: Trial 3

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]