Coding

Part:BBa_K2083005

Designed by: Virginia Massa   Group: iGEM16_WPI_Worcester   (2016-10-14)


ADAR1 3XTEN

Coding region for ADAR1, an enzyme that performs A to I edits in RNA with a 3xXTEN (144 nucleotide) linker. The one extension linker sequence is TCTGGCTCAGAAACGCCCGGTACGTCCGAGTCTGCAACACCAGAAAGC. The 3xXTEN is the one extension sequence tripled. ADAR1 with a 1xXTEN is BBa_K2083003, and the 2xXTEN can be found here BBa_K2083004. I, Inosine, is read as a G.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 98
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1017


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Categories
Parameters
None