Coding

Part:BBa_K2083002

Designed by: Virginia Massa   Group: iGEM16_WPI_Worcester   (2016-10-14)


APOBEC 3XTEN

Coding region for APOBEC1, an enzyme that performs C to U edits on DNA and RNA with a 3xXTEN (144 nucleotide) linker. The one extension linker sequence is TCTGGCTCAGAAACGCCCGGTACGTCCGAGTCTGCAACACCAGAAAGC. The 3xXTEN is the one extension sequence tripled. APOBEC with a 1xXTEN is BBa_K2083000, and the 2xXTEN can be found here BBa_K2083001.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None