Coding

Part:BBa_K2082125

Designed by: Cassandra Königs   Group: iGEM16_Bielefeld-CeBiTec   (2016-10-13)


Stop Beta-Lactamase (K30*)

Coding sequence of a beta-lactamase with an artificial point mutation (K30*).
The part was used in the device BBa_K2082151. This complex was used to test our in vivo mutagenesis systems (BBa_K2082106, BBa_K2082117).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 716


[edit]
Categories
Parameters
None