Coding

Part:BBa_K2082124

Designed by: Carsten Hain   Group: iGEM16_Bielefeld-CeBiTec   (2016-10-21)


seqA

Biology

SeqA is a GATC-binding protein, which also controls the methylation state of DNA. It counteracts Dam by demethylating DNA. This results in hypomethylated DNA on both strands, which further decreases the efficiency of DNA mismatch repair (Yang et al. 2004).

Application

BBa_K2082124 was used in our genome wide mutator BBa_K2082117, increasing the mutating capabilities of dam BBa_K1500994.
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

  • Yang, Hanjing; Wolff, Erika; Kim, Mandy; Diep, Amy; Miller, Jeffrey H. (2004): Identification of mutator genes and mutational pathways in Escherichia coli using a multicopy cloning approach. In Molecular microbiology 53 (1), pp. 283–295. DOI: 10.1111/j.1365-2958.2004.04125.x.


[edit]
Categories
Parameters
None