Generator

Part:BBa_K2082111

Designed by: Carsten Hain   Group: iGEM16_Bielefeld-CeBiTec   (2016-10-14)


Assembly of six mutator genes, each with weak promoter; terminal terminator

Assembly of dnaQ926 (BBa_K1333108), dam (BBa_K1500994), seqA, emrR (BBa_K1500995), ugi (BBa_K2082104) and cda1 (BBa_K2082105), each with weak RBS BBa_B0031.


This part was cloned downstream of BBa_K2082112 to obtain the functional and characterized BBa_K2082117

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 194
    Illegal BamHI site found at 1081
    Illegal XhoI site found at 2663
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 66
    Illegal AgeI site found at 555
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3325


[edit]
Categories
Parameters
None