Generator

Part:BBa_K2082110

Designed by: Carsten Hain   Group: iGEM16_Bielefeld-CeBiTec   (2016-10-14)


Weak RBS - dnaQ926 cds - terminator

Assembly of the mutator dnaQ926 (BBa_K1333108) with weak RBS BBa_B0031 and terminator.


This part was cloned downstream of BBa_K2082112 to obtain the functional and characterized BBa_K2082116

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 194
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 66
    Illegal AgeI site found at 555
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None