Protein_Domain
Part:BBa_K2082007
Designed by: Mikail Sahin Group: iGEM16_Bielefeld-CeBiTec (2016-10-14)
Zika E-Protein
Zika E Protein
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1134
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 808
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1503
With this part an E. coli codon optimized E protein of the Zika virus (ZIKV) strain Mr 766 is made available for the use in this competition. It was designed after the construct took from Uniprot (A0A060H177).
Figure 1: Gelelectrophoresis of the ZIKV E Protein. After cloning and PCR-amplification with specific primers the sample was loaded onto an 1% agarose gel. Order from left to right: Sample with ZIKV E protein codon, 1kb DNA Ladder (by New England Biolabs)
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Categories
Parameters
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