Regulatory

Part:BBa_K208010

Designed by: Elisabeth Linton   Group: iGEM09_Utah_State   (2009-10-12)

Lac Promoter (BBa_R0010) and RBS (B0034)

This part is a composite of the Lac promoter (BBa_R0010) and a ribosome-binding site (BBa_B0034). The purpose of this part is to simplify the construction of BioBrick parts. This part can be used when it is not necessary to test multiple ribosome-binding sites, and the high-efficiency BBa_B0034 part will suffice. It is functional and IPTG induced. Please refer to the original pages on these two different parts for more extensive details on their functionality.


Usage and Biology

The IPTG inducible lac promoter is advantageous for use in many biological applications. It is a commonly used promoter, and IPTG inducibility provides the user with control over the expression of their genetic system.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None