Device
Part:BBa_K2080008
Designed by: Haojian Li Group: iGEM16_SCUT-China_B (2016-10-13)
bax-sgRNA4
This guide RNA is a hundred base-long molecule with a dCas9-VP64 scaffold sequence, which can guide dCas9-VP64 to its complementary part of a double-strand DNA. In our project, this gRNA is targeting to the promoter of bax, a proapoptosis protein, which can activate the transcription of bax.
Usage and Biology
gRNA sequence is complementary to the promoter of bax, 978 bp upstream of the transcription start site.
Characterization
This part was designed for dCas9-VP64 targetting bax promoter then upregulated the transcription level of bax. The transcription level of bax is detected by real-time PCR(Fig.1).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |