Device

Part:BBa_K2080001

Designed by: Haojian Li   Group: iGEM16_SCUT-China_B   (2016-09-22)


bax-sgRNA1

This guide RNA is a hundred base-long molecule with a dCas9-VP64 scaffold sequence, which can guide dCas9-VP64 to its complementary part of a double-strand DNA. In our project, this gRNA is for dCas9-VP64 targeting to the promoter of bax, which can activate the transcription of bax. Besides, this guide RNA is complementary to 3 different loci in the synthetic promoter, Bax-1, and gRNA-dCas9-VP64 complex can promote transcription downstream of a synthetic promoter.

Usage and Biology

This guide RNA is for dCas9-VP64 targeting bax promoter and Bax-1 promoter.

Charaterization

This part was designed for the tumor-specific promoter-based CRISPR-mediated transcription activation.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None