Part:BBa_K2077001
pSB413-GPD: HIS3-selectable S. cerevisiae Expression Vector (RFC 10)
This is a RFC10 standard yeast expression vector adapted from the widely used plasmid pSB413-GPD. The MCS of p413-GPD was replaced with the standard BioBrick prefix and suffix and an illegal PstI restriction site near the HIS3 promoter of p413-GPD was removed. In the resultant plasmid, pSB413-GPD (5828 bp), the BioBrick prefix and suffix is situated between the strong constitutive yeast GPD promoter (TDH3) and CYC1 terminator allowing for facile cloning and expression of translational units in yeast. Standard VF2 and VR primer binding sites are included and M13 primer binding sites bracket the promoter and terminator. The plasmid has a ColE1 origin and ampicillin resistance for replication in E. coli. It also contains a yeast CEN/ARS and HIS3-selectable marker to provide high fidelity maintenance and low copy number in S. cerevisiae.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 5807
Illegal NheI site found at 1343
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 5813 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 5807
Illegal BglII site found at 1392
Illegal BglII site found at 1452
Illegal XhoI site found at 5148 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 5807
Illegal suffix found at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 5807
Plasmid lacks a suffix.
Illegal XbaI site found at 5822
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NgoMIV site found at 603 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 3785
Illegal SapI.rc site found at 2763
Illegal SapI.rc site found at 4867
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