Part:BBa_K2075009
TAL-effector Hax3-2xNN
This part synthesizes a transcription activator like (TAL) effector protein. They were found in Xanthomonas bacteria. These bacteria are plant pathogenes and infects plants via a secretion typ III system. In the plant cells these proteins binds promoter sites. This leads to a transcriptional activation of the genes behind the promoter. The protein contains tandem repeats up to 34.5. Each repeat binds one nucleotide of the target sequence. The 12th and 13th amino acid of each repeat leads to the specific binding of the DNA sequence. We circularised the TAL-effectors with the help of Inteins at the N- and C-terminus of each TAL-effector.
The 12th and 13th amino acid of each of the 11.5 repeats from our TAL Hax3 â 2xNN are: NI HD NI HD HD HD NN NN NS HD NI
They bind the DNA sequence: A C A C C C G/A G/A N C A.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1743
- 1000COMPATIBLE WITH RFC[1000]
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