Coding

Part:BBa_K2074998

Designed by: Ruicheng Dai   Group: iGEM16_FAFU-CHINA   (2016-10-14)


cry11Aa suit for Chlamydomonas reinhardti

This part codes for the protein cry11Aa which from Bacillus thuringiensis and is toxic to the larvae of mosquitoes. Our project aim to express such protein in Chlamydomonas reinhardti to kill the larve of mosquitoes. To express this protein in Chlamydomonas reinhardti we optimizate the codon.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1
    Illegal PstI site found at 1943
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1943
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1
    Illegal PstI site found at 1943
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1
    Illegal PstI site found at 1943
    Illegal NgoMIV site found at 523
    Illegal NgoMIV site found at 1687
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 55
    Illegal BsaI.rc site found at 250
    Illegal BsaI.rc site found at 1465
    Illegal SapI.rc site found at 1446


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Parameters
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