Coding

Part:BBa_K2074101

Designed by: Hao Feng   Group: iGEM16_FAFU-CHINA   (2016-10-11)


Cry4Aa from Bacillus thuringiensis serovar israelensis strain BRC-LLP29

Bacillus thuringiensis serovar israelensis strain BRC-LLP29 putative cry4A gene, complete cds；GenBank: JQ228565.1

Fig.1 The tertiary structure of Cry4Aa toxin from Bacillus thuringiensis serovar israelensis strain BRC-LLP29

800px-T--FAFU-CHINA--partsclones.png


Part BBa_K42415005(https://parts.igem.org/Part:BBa_K2415005) is a standardized and codon-optimized version of this part and it is suitable for protein expression in E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1638
    Illegal EcoRI site found at 1766
    Illegal SpeI site found at 612
    Illegal SpeI site found at 2502
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1638
    Illegal EcoRI site found at 1766
    Illegal SpeI site found at 612
    Illegal SpeI site found at 2502
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1638
    Illegal EcoRI site found at 1766
    Illegal BglII site found at 912
    Illegal XhoI site found at 980
    Illegal XhoI site found at 1799
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1638
    Illegal EcoRI site found at 1766
    Illegal SpeI site found at 612
    Illegal SpeI site found at 2502
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1638
    Illegal EcoRI site found at 1766
    Illegal SpeI site found at 612
    Illegal SpeI site found at 2502
    Illegal NgoMIV site found at 2625
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None