Composite

Part:BBa_K2073013

Designed by: WEI-CHIA HSU   Group: iGEM16_NTHU_Taiwan   (2016-10-14)


CrcB-La2 with IPTG promoter

Excess fluoride is toxic for bacteria. After the degradation of PFOA, fluoride would be released simultaneously. To prevent the E. coli killed by excess fluoride, we transfected Crcb(La2) gene into E. Coli.
Crcb(La2) is one of fluoride transporters - integration with the plasma membrane - able to expulse fluoride from E. Coli.
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 422
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None