Composite
Part:BBa_K2073012
Designed by: WEI-CHIA HSU Group: iGEM16_NTHU_Taiwan (2016-10-14)
CrcB-La1 with IPTG promoter
Excess fluoride is toxic for bacteria. After the degradation of PFOA, fluoride would be released simultaneously. To prevent the E. coli killed by excess fluoride, we transfected Crcb(La1) gene into E. Coli.
Crcb(La1) is one of fluoride transporters - integration with the plasma membrane - able to expulse fluoride from E. Coli.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 320
Illegal AgeI site found at 416
Illegal AgeI site found at 587 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 312
[edit]
Categories
Parameters
None |