Composite

Part:BBa_K2073012

Designed by: WEI-CHIA HSU   Group: iGEM16_NTHU_Taiwan   (2016-10-14)


CrcB-La1 with IPTG promoter

Excess fluoride is toxic for bacteria. After the degradation of PFOA, fluoride would be released simultaneously. To prevent the E. coli killed by excess fluoride, we transfected Crcb(La1) gene into E. Coli.
Crcb(La1) is one of fluoride transporters - integration with the plasma membrane - able to expulse fluoride from E. Coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 320
    Illegal AgeI site found at 416
    Illegal AgeI site found at 587
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 312


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Categories
Parameters
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